P-01/CO-01 : Gene polymorphism in essential hypertension & its relation to environmental factors in North Indians

posted Jan 26, 2017, 8:43 AM by sourav ghosh
Qulsoom Naz, Narsingh Verma, M. Serajuddin, A.A.Mahdi, M.L.Patel 
King George's Medical University, Lucknow, India

Aim- We aimed to determine genotype and allele frequencies of angiotensinogen (AGT) and alpha adducing (ADD1) polymorphisms in patients with essential hypertension.
Methods- We recruited total 205 subjects. In which 105 were essential hypertensive and 100 were Healthy controls. DNA samples for each individual were isolated from peripheral blood by standard phenol /chloroform method analyzed by polymerase chain reaction & enzymatic digestion. Lipid profile was analyzed by VITROS ̊ 250 Dry Biochemistry Fully auto-analyzer (Gonson & Gonson Company). Electrolytes (in serum & urine) were measured by ion-selective electrodes (Roche Hitachi MODULAR, Hitachi Ltd) in Clinical lab of Biochemistry.
Results- The distribution for each ADD1 genotypes were 61.96% for GG (69), 33.51% for GT (21) and 4.53% for TT(11) in the essential hypertensive group; 82.72% for GG (91), 16.46% for GT (9) and 0.82% for TT in the control group. The distribution of AGT genotypes was found significantly different between groups (x2 = 10.00: df = 2; P = 0. 006).
The frequencies for each of the AGT genotypes were found as 44.66% for MM (43), 44.33% for GT (49), and 11% for TT (9) in essential hypertensive group; 64% for MM (66), 32% for GT (36), and 4% for TT (3) in healthy control group. The distribution of AGT genotypes did not highly significant as compared to AGT between the groups. We suggest that AGT and ADD1 gene polymorphism play a role for development of essential hypertension (x2 = 9.767: df = 2; P = 0.007). 
Conclusion- Patients with essential hypertension exhibited higher levels of Serum cholesterol, LDL Cholesterol & TG than in control subjects. Taken together the genotype and biochemical parameters & considering the restrictive selection criteria used, the present results suggest a relationship between these gene polymorphism and essential hypertension in North Indians.